Product Name :
hnRNP U Recombinant Rabbit Monoclonal Antibody [JB23-43]
Predicted band size :
91 kDa
Observed band size :
120 kDa ET7107-10, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).” style=”width: auto”> ICC staining of hnRNP U in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-10, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). ET7107-10, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).” style=”width: auto”> ICC staining of hnRNP U in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-10, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). ET7107-10, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).” style=”width: auto”> ICC staining of hnRNP U in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-10, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). ET7107-10) at 1/400 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-10) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.” style=”width: auto”> Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-hnRNP U antibody (ET7107-10) at 1/400 dilution.
Synonyms:
Heterogeneous nuclear ribonucleoprotein U antibody hnRNP U antibody hnRNP U protein antibody HNRNPU antibody hnRNPU protein antibody HNRPU antibody HNRPU_HUMAN antibody p120 antibody p120 nuclear protein antibody pp120 antibody 展开
Function :
Heterogeneous nuclear ribonucleoprotein U is a protein that in humans is encoded by the HNRNPU gene.tert-Butyl (3-oxocyclopentyl)carbamate structure This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs).36902-22-4 Data Sheet The hnRNPs are RNA binding proteins that form complexes with heterogeneous nuclear RNA (hnRNA).PMID:33499659 These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene contains a RNA binding domain and scaffold-associated region (SAR)-specific bipartite DNA-binding domain. This protein is also thought to be involved in the packaging of hnRNA into large ribonucleoprotein complexes. During apoptosis, this protein is cleaved in a caspase-dependent way. Cleavage occurs at the SALD site, resulting in a loss of DNA-binding activity and a concomitant detachment of this protein from nuclear structural sites. But this cleavage does not affect the function of the encoded protein in RNA metabolism. At least two alternatively spliced transcript variants have been identified for this gene.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Recombinantpeptide within Human hnRNP U aa 1-140 / 825.
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IF-Cell, IHC-P
Coupling :
unconjugated
Clone number:
JB23-43
RRID:
AB_3070726
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 91 kDa
Positive control :
NIH/3T3 cell lysate, SH-SY5Y cell lysate, K562 cell lysate, A431, HepG2, SH-SY5Y, rat brain tissue, human tonsil tissue, human lung carcinoma tissue, human kidney tissue, mouse colon tissue, human cervix tissue, human thyroid tissue, human colon carcinoma tissue, human skin tissue, human breast tissue, human breast carcinoma tissue, human esophagus tissue, human placenta tissue, mouse bladder tissue.